Please use this identifier to cite or link to this item: http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/10023
Title: Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification(AI-LAMP) for Rapid Detection of SARS-CoV-2
Authors: Rohaim, M.A.
Clayton, E.
Sahin, I.
Julianne, V.
Khalifa, M.E.
Al-Natour, M.Q.
Bayoumi, M.
Poirier, M.C.
Branavan, M.
Mukunthan, T.
Chaudhry, N.S.
Ravinder, S.
Amy, B.
Peter, B.
Hacking, W.
Botham, J.
Boyce, J.
Wilkinson, H.
Williams, C.
Whittingham-Dowd, J.
Shaw, E.
Matt Hodges
Lisa Butler
Bates, M.D.
Roberto La, R.
Balachandran, V.
Anil, F.
Munir, M.
Keywords: SARS-CoV-2;Diagnosis;LAMP;Point of care;Artificial intelligence
Issue Date: 2020
Publisher: MDPI
Abstract: Untilvaccinesandeffectivetherapeuticsbecomeavailable,thepracticalsolutiontotransit safelyoutofthecurrentcoronavirusdisease19(CoVID-19)lockdownmayincludetheimplementation ofaneffectivetesting,tracingandtrackingsystem.However,thisrequiresareliableandclinically validateddiagnosticplatformforthesensitiveandspecificidentificationofSARS-CoV-2.Here, wereportonthedevelopmentofadenovo,high-resolutionandcomparativegenomicsguided reverse-transcribedloop-mediatedisothermalamplification(LAMP)assay.Tofurtherenhancethe assayperformanceandtoremoveanysubjectivityassociatedwithoperatorinterpretationofresults, weengineeredanovelhand-heldsmartdiagnosticdevice.Therobustdiagnosticdevicewasfurther furnishedwithautomatedimageacquisitionandprocessingalgorithmsandthecollateddatawas processedthroughartificialintelligence(AI)pipelinestofurtherreducetheassayruntimeandthe subjectivityofthecolorimetricLAMPdetection.ThisadvancedAIalgorithm-implementedLAMP (ai-LAMP)assay,targetingtheRNA-dependentRNApolymerasegene,showedhighanalytical sensitivityandspecificityforSARS-CoV-2.Atotalof~200coronavirusdisease(CoVID-19)-suspected NHSpatientsamplesweretestedusingtheplatformanditwasshowntobereliable,highlyspecific andsignificantlymoresensitivethanthecurrentgoldstandardqRT-PCR.Therefore,thissystemcould provideanefficientandcost-effectiveplatformtodetectSARS-CoV-2inresource-limitedlaboratories.
URI: http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/10023
DOI: doi:10.3390/v12090972
Appears in Collections:Electrical & Electronic Engineering



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