Assessing the evolution of Sri Lankan sandfly species belong to genus Sergentomyia using cytochrome C Oxidase subunit I (CO 1) partial sequence

Abstract

Sandflies are vectors of Leishmania parasites that cause Leishmaniasis in human. The taxonomy of sandflies and their vector potential have not been comprehensively documented. This study aimed to assess the evolution of the sandflies by using a mitochondrial DNA marker. Partial DNA sequence for cytochrome c oxidase subunit 1 of Sri Lankan sandfly species were used for the analysis. Edited sequences were used in the phylogeny tree construction using Maximum likelihood method. The consensus tree was obtained with condensation of the clades at a cut-off value of 70% of the bootstrapping value. The phylogenetic tree obtained corresponds to the traditional taxonomy where Se.bailyi deviates from Subgenus Parratomyia and form a separate group, as discussed in some taxonomic keys. The tree also supports the tritomy concept of the subgenera Parratomyia, Sergentomyia, and Neophlebotomus. It also reflects the species complex of Se.babu insularis and Se.babu babu within the respective subgenus; Parratomyia. Phylogenetic relationship among the species belong to the genus Sergentomyia is reassessed. Combined approach of molecular phenotypes (identified via DNA markers) together with morphological features is suggested to identify the definitive taxonomic status of species.