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http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/9114
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DC Field | Value | Language |
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dc.contributor.author | Surendran, S.N. | - |
dc.contributor.author | Truelove, N. | - |
dc.contributor.author | Sarma, D.K. | - |
dc.contributor.author | Jude, P.J. | - |
dc.contributor.author | Ramasamy4, R. | - |
dc.contributor.author | Gajapathy, K. | - |
dc.contributor.author | Peiris, L.B.S. | - |
dc.contributor.author | Parakrama Karunaratne, S.H.P. | - |
dc.contributor.author | Walton, C. | - |
dc.date.accessioned | 2023-02-14T08:14:17Z | - |
dc.date.available | 2023-02-14T08:14:17Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/9114 | - |
dc.description.abstract | The identification of species B and E in the Anopheles culicifacies complex in the Indian subcontinent has been based on Y-chromosome karyotype. Since no detectable variations were previously found in DNA markers commonly used for sibling species identification, further molecular characterization using cytochrome oxidase subunit I (COI) and microsatellite markers was carried out on Y-chromosome karyotyped Anopheles culicifacies specie B and E from Unnichchai, Kallady and Ranawarunawa in Sri Lanka. Findings: COI sequence analysis (n = 22) revealed the presence of nine unique haplotypes with six in each species. Three haplotypes were shared by both species. The two sibling species had a pairwise FST value of 1.338 (p < 0.05) with the number of migrants (Nm) value <1. The genetic structure analysis resulted in two genetic clusters not 100 % associated with karyotypes. While none of the species B were incorrectly assigned two were inconclusive. Five out of 26 specimens karyotyped as species E were incorrectly assigned, while further 9 were inconclusive. Conclusions: The new molecular data support the existence of two genetically different populations of the Culicifacies Complex in Sri Lanka that are not associated with the Y-chromosome karyotype. Detailed analysis with more microsatellite markers and assortative mating experiments are needed to e | en_US |
dc.language.iso | en | en_US |
dc.publisher | Parasites & Vectors | en_US |
dc.subject | Anopheles culicifacies | en_US |
dc.subject | COI | en_US |
dc.subject | Malaria | en_US |
dc.subject | Microsatellite | en_US |
dc.subject | Mosquito vector | en_US |
dc.subject | Species complex | en_US |
dc.subject | Sri Lanka | en_US |
dc.subject | Y-chromosome karyotype | en_US |
dc.title | Karyotypic assignment of Sri Lankan Anopheles culicifacies species B and E does not correlate with cytochrome oxidase subunit I and microsatellite genotypes | en_US |
dc.type | Article | en_US |
Appears in Collections: | Zoology |
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File | Description | Size | Format | |
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Karyotypic assignment of Sri Lankan Anopheles culicifacies species B and E does not correlate with cytochrome oxidase subunit I and microsatellite genotypes.pdf | 2.01 MB | Adobe PDF | View/Open |
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