Please use this identifier to cite or link to this item: http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/9114
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dc.contributor.authorSurendran, S.N.-
dc.contributor.authorTruelove, N.-
dc.contributor.authorSarma, D.K.-
dc.contributor.authorJude, P.J.-
dc.contributor.authorRamasamy4, R.-
dc.contributor.authorGajapathy, K.-
dc.contributor.authorPeiris, L.B.S.-
dc.contributor.authorParakrama Karunaratne, S.H.P.-
dc.contributor.authorWalton, C.-
dc.date.accessioned2023-02-14T08:14:17Z-
dc.date.available2023-02-14T08:14:17Z-
dc.date.issued2015-
dc.identifier.urihttp://repo.lib.jfn.ac.lk/ujrr/handle/123456789/9114-
dc.description.abstractThe identification of species B and E in the Anopheles culicifacies complex in the Indian subcontinent has been based on Y-chromosome karyotype. Since no detectable variations were previously found in DNA markers commonly used for sibling species identification, further molecular characterization using cytochrome oxidase subunit I (COI) and microsatellite markers was carried out on Y-chromosome karyotyped Anopheles culicifacies specie B and E from Unnichchai, Kallady and Ranawarunawa in Sri Lanka. Findings: COI sequence analysis (n = 22) revealed the presence of nine unique haplotypes with six in each species. Three haplotypes were shared by both species. The two sibling species had a pairwise FST value of 1.338 (p < 0.05) with the number of migrants (Nm) value <1. The genetic structure analysis resulted in two genetic clusters not 100 % associated with karyotypes. While none of the species B were incorrectly assigned two were inconclusive. Five out of 26 specimens karyotyped as species E were incorrectly assigned, while further 9 were inconclusive. Conclusions: The new molecular data support the existence of two genetically different populations of the Culicifacies Complex in Sri Lanka that are not associated with the Y-chromosome karyotype. Detailed analysis with more microsatellite markers and assortative mating experiments are needed to een_US
dc.language.isoenen_US
dc.publisherParasites & Vectorsen_US
dc.subjectAnopheles culicifaciesen_US
dc.subjectCOIen_US
dc.subjectMalariaen_US
dc.subjectMicrosatelliteen_US
dc.subjectMosquito vectoren_US
dc.subjectSpecies complexen_US
dc.subjectSri Lankaen_US
dc.subjectY-chromosome karyotypeen_US
dc.titleKaryotypic assignment of Sri Lankan Anopheles culicifacies species B and E does not correlate with cytochrome oxidase subunit I and microsatellite genotypesen_US
dc.typeArticleen_US
Appears in Collections:Zoology



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